Figurations of Time in Asia

The experience and the ensuing structuring of time forms a constitutive part of human cultures. There are many ways of coming to terms with time, calendars and historiographies being its most common cultural representations. The contributions to this volume deal with lesser known figurations that result directly from the various perceptions about time and phenomena related to time. Diachronous investigations in various parts of Asia (predominantly South Asia) reveal a broad spectrum of such visual and literary figurative manifestations. While Hinduism recognizes a divine personification of time and allocates the ominous factor time in an ontological proximity to death, other cultures of Asia have developed their own specific concepts and strategies. This collection of essays combines perspectives of various disciplines on figurations in which time congeals, as it were. These figurations result from local time regimes, and beyond demonstrating their diversity of forms this volume offers coordinates for a comparison of cultures. The topics include chronograms as well as early Buddhist topoi of the vastness of time, the Indian Jaina representation of both temporality and non-temporality and the teachings of a Mediaeval Zen master hinting at the more stationary aspects of time

Update kosten en baten van harmonisatie van de rechtspositie van overheidspersoneel

Dit rapport beschrijft de kosten en baten voor de overheid als ambtenaren dezelfde rechtspositie krijgen als andere werknemers (‘harmonisatie’). Zij krijgen dan een arbeidscontract in plaats van een aanstelling; gaan voor rechtszaken naar de kantonrechter in plaats van de bestuursrechter; en krijgen een eigen CAO. Omdat er bij verschillende kosten en baten onzekerheid bestaat, wordt gewerkt met drie scenario’s. De kosten van harmonisatie zijn eenmalig en liggen tussen € 53 en 186 miljoen. Dit betreft met name opleidingen en administratieve organisatie. De baten zijn € 6,6 tot 21,9 miljoen per jaar. Deze baten bestaan uit besparingen bij de rechtspraak en besparingen door deregulering. In het middenscenario is de terugverdientijd ruim zeven jaar. In het meest ongunstige geval loopt de terugverdientijd op tot 28 jaar. Bij een goede planning en sturing van het proces - waarbij de kosten in de hand worden gehouden - en baten die gunstig uitvallen, is de investering in drie jaar terugverdiend. Dit rapport bouwt voort op een rapport van SEO en Regioplan uit 2006. De kosten zijn gedaald omdat de huidige inzichten wijzen op minder behoefte aan opleidingen. De baten zijn hoger door een betere inschatting van de opbrengsten van de overgang van bestuursrechter naar kantonrechter

Riddles of the Rock-Carved Hindu and Jaina Sculptures and Inscriptions at Rakhetrā

The article presents new evidence on the archaeological site of Rakhetrā (M.P.), with particular attention to the rock-carved Hindu and Jaina sculptures and inscriptions. Photos of the three 10th, 15th and 17th century inscriptions on site are published for the first time, as well as a tentative transliteration of the important Jaina inscription next to a foot-image of Upādhyāya Viśālarāja, listing the names of his yet unidentified 15th century Jaina monastic lineage. The functions of rock-cut Jaina sculptures at remote places, object for worship, ornamentation or propagation, are discussed, and it is argued that the colossal sculpture of Ādinātha, that dominates the multi-functional site, was probably primarily a marker of the religious sphere of influence of the Digambara tīrtha Thūbonjī

Анализ эффективности применения технологии гидравлического разрыва пласта на нефтяных месторождениях Западной Сибири

Анализ технологических и геологических особенностей применения гидравлического разрыва пласта. Эффективность применения ГРП путем сравнения показателей разработки до и после введения данного метода на нефтяных месторождениях Западной Сибири.Analysis of technological and geological features of the use of hydraulic fracturing. The effectiveness of hydraulic fracturing by comparing the development indicators before and after the introduction of this method in oil fields of Western Siberia

Allelic Heterogeneity at the Equine KIT Locus in Dominant White (W) Horses

White coat color has been a highly valued trait in horses for at least 2,000 years. Dominant white (W) is one of several known depigmentation phenotypes in horses. It shows considerable phenotypic variation, ranging from ∼50% depigmented areas up to a completely white coat. In the horse, the four depigmentation phenotypes roan, sabino, tobiano, and dominant white were independently mapped to a chromosomal region on ECA 3 harboring the KIT gene. KIT plays an important role in melanoblast survival during embryonic development. We determined the sequence and genomic organization of the ∼82 kb equine KIT gene. A mutation analysis of all 21 KIT exons in white Franches-Montagnes Horses revealed a nonsense mutation in exon 15 (c.2151C>G, p.Y717X). We analyzed the KIT exons in horses characterized as dominant white from other populations and found three additional candidate causative mutations. Three almost completely white Arabians carried a different nonsense mutation in exon 4 (c.706A>T, p.K236X). Six Camarillo White Horses had a missense mutation in exon 12 (c.1805C>T, p.A602V), and five white Thoroughbreds had yet another missense mutation in exon 13 (c.1960G>A, p.G654R). Our results indicate that the dominant white color in Franches-Montagnes Horses is caused by a nonsense mutation in the KIT gene and that multiple independent mutations within this gene appear to be responsible for dominant white in several other modern horse populations

Screening of DUB activity and specificity by MALDI-TOF mass spectrometry

Deubiquitylases (DUBs) are key regulators of the ubiquitin system which cleave ubiquitin moieties from proteins and polyubiquitin chains. Several DUBs have been implicated in various diseases and are attractive drug targets. We have developed a sensitive and fast assay to quantify in vitro DUB enzyme activity using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Unlike other current assays, this method uses unmodified substrates, such as diubiquitin topoisomers. By analyzing 42 human DUBs against all diubiquitin topoisomers we provide an extensive characterization of DUB activity and specificity. Our results confirm the high specificity of many members of the OTU and JAMM DUB families and highlight that all USPs tested display low linkage selectivity. We also demonstrate that this assay can be deployed to assess the potency and specificity of DUB inhibitors by profiling 11 compounds against a panel of 32 DUBs

The E3 ubiquitin ligase SCF(Fbxo7) mediates proteasomal degradation of UXT isoform 2 (UXT-V2) to inhibit the NF-κB signaling pathway.

BACKGROUND: Ubiquitously eXpressed Transcript isoform 2 (UXTV2) is a prefoldin-like protein involved in NF-κB signaling, apoptosis, and the androgen and estrogen response. UXT-V2 is a cofactor in the NF-κB transcriptional enhanceosome, and its knockdown inhibits TNF-α -induced NF-κB activation. Fbxo7 is an F-box protein that interacts with SKP1, Cullin1 and RBX1 proteins to form an SCF(Fbxo7) E3 ubiquitin ligase complex. Fbxo7 negatively regulates NF-κB signaling through TRAF2 and cIAP1 ubiquitination. METHODS: We combine co-immunoprecipitation, ubiquitination in vitro and in vivo, cycloheximide chase assay, ubiquitin chain restriction analysis and microscopy to investigate interaction between Fbxo7 and overexpressed UXT-V2-HA. RESULTS: The Ubl domain of Fbxo7 contributes to interaction with UXTV2. This substrate is polyubiquitinated by SCF(Fbxo7) with K48 and K63 ubiquitin chain linkages in vitro and in vivo. This post-translational modification decreases UXT-V2 stability and promotes its proteasomal degradation. We further show that UXTV1, an alternatively spliced isoform of UXT, containing 12 additional amino acids at the N-terminus as compared to UXTV2, also interacts with and is ubiquitinated by Fbxo7. Moreover, FBXO7 knockdown promotes UXT-V2 accumulation, and the overexpression of Fbxo7-ΔF-box protects UXT-V2 from proteasomal degradation and enhances the responsiveness of NF-κB reporter. We find that UXT-V2 colocalizes with Fbxo7 in the cell nucleus. CONCLUSIONS: Together, our study reveals that SCF(Fbxo7) mediates the proteasomal degradation of UXT-V2 causing the inhibition of the NF-κB signaling pathway. GENERAL SIGNIFICANCE: Discovering new substrates of E3 ubiquitin-ligase SCF(Fbxo7) contributes to understand its function in different diseases such as cancer and Parkinson.Biotechnology and Biological Science Research Council [BB/J007846/1]

Measurement of the Bˉ→Dℓνˉ\bar{B}\to D\ell\bar{\nu} Partila Width and Form Factor Parameters

We have studied the decay $\bar{B} \to D\ell\bar{\nu}$, where $\ell=e or \mu$. From a fit to the differential decay rate $d\Gamma/dw$ we measure the rate normalization ${\cal F}_D(1)|V_{cb}|$ and form factor slope $\hat{\rho}^2_D$, and, using measured values of $\tau_B$, find $\Gamma(\bar{B} \to D\ell\bar{\nu}) = (12.0 \pm 0.9 \pm 2.1) ns^{-1}$. The resulting branching fractions are ${\cal B}(\bar{B}^0 \to D^+\ell^-\bar{\nu})=(1.87 \pm 0.15 \pm 0.32)$ and ${\cal B}(B^- \to D^0\ell^-\bar{\nu})=(1.94 \pm 0.15 \pm 0.34)$. The form factor parameters are in agreement with those measured in $\bar{B} \to D^*\ell\bar{\nu}$ decays, as predicted by heavy quark effective theory.Comment: 11 pages, postscript file also available through http://w4.lns.cornell.edu/public/CLN